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Someone in my group chat poster the picture and I can’t unsee it. It’s horrible.
Someone in my group chat poster the picture and I can’t unsee it. It’s horrible.
My alignment is neutral good
Im the one that was paying for Netflix for my family, but the password crack down motivated me to learn how to build a server and go full arrs. They had a good thing going, but now that $26 a month will be used to buy hdds.
I just wanted to see Luke in the role of obi wan or Yoda after decades of Jedi training be a hero and pass on his legacy by training the next generation. But I would have been ok with one heroic lightsaber battle, and a reunion with han, chewy and Leia.
Watching the last Jedi and seeing someone who tossed his blade away because he saw the good in essentially “Space Hitler” try to kill his own nephew because he was having a nightmare so out of character. Then having him overdose on the force and die like a chump, broke me. I left the theater in silence. The last Jedi is also the only star wars movie with out a light saber fight. No blades ever touched.
A partial solution is to remove nimby laws that prevent accessory dwelling units, duplexes, and medium and high density housing. Then you use the tax laws to heavily punish corporate ownership of low and medium density housing, and make it progressively more expensive for mom and pop landlords after a reasonable number of properties.
Hopefully this would lower the cost for single family housing, while increasing supply and variety of rentals. But somehow you’d have to prevent end stage capitalist collusion from fixing prices so competition could actually work to drive down prices.
Lol yeh but it’s partially funded by money saved from canceled subscriptions.
I’m a noob, I started 2 months ago with immich and tailscale. Now I have an unraid server, it’s a slippery slope.
Pretty good description. Few extra points. -you don’t need to don’t need to do PCR to have enough Nucleic acids to visualize on the gel. Often you use gels to visualize things like purified plasmids. You can also digest your plasmids with enzymes to confirm genomic insert. -these type of gels have many uses outside of direct comparisons, for example you can use the gel purity DNA of a particular size by cutting out the band, melting the gel and using a silica column to pull the DNA out of the melted gel slice. This is super common when you do molecular cloning.
For what is possibly wrong with the top gel.
-the blurred bands can be a lot of things. It might just be inherited to the sample type, for example raw genomic DNA will look like this because you’ll have enzyme that degrade DNA, and a lot of mid replication chromosomes resulting in fragments existing across a spectrum of sizes equally. Additionally the it could also be user error with overloaded well or not enough loading buffer in the sample so it’s not pushed in to gel evenly, or it was run to fast.
Overall the top gel just looks like crap and the bottom gel looks great, but depending on what was run you really don’t know what the gel should look like, if I was testing endonuclease activity the top gel would probably be what I was hoping to get. If was doing PCR on single gene, the bottom one is better.
I paid $1000 for books my first semester of college back in 2007. I felt so burnt and violated I never bought another textbook. I made it through the rest of undergrad, a masters, and a PhD in biochemistry by checking out books from the library, borrowing textbooks from friends, and going sailing. When I taught I made it a point to teach my students about all the ways they can avoid becoming a victim like myself.
I’m a biochemist
And the magic is a hot plate and a spectroscope. The top two beige boxes do the exact same thing qpcr.
The everything is woke or not is out of control, when the real wide spread problem is just bad writing and story telling. I’m just tired of stories with contrived conflicts that occur just to make the story move forward “ie the entire plot of God of war Ragnarok”, and established characters making uncharacteristic choices and actions without any development towards that change, “ie Luke Skywalker in the last Jedi and basically everyone in the last season of game of thrones”.
I’m from Chicago, I lived in Pittsburgh for 7 years. Best pizza in Pittsburgh was Domino’s.
I got a 26 while infecting the entire room with the flu, because the ACT is also the Prairie state achievement exam required for graduation in Illinois and there are no make ups.
HiFi rush is basically a devil may cry rhythm game. Hell devil may cry itself is basically a rhythm game when trying to get SSS ranks.
Wait until they learn the Spanish word for black, or worse Latin.